This Scantool uses SAEJ1979 (ODB II) protocol on ISO9141, ISO14230, SAEJ1850 VPW and SAEJ1850-PWM interfaces depending on what hardware interface is attached to the PC. The scantool does not yet support any manufacturer specific protocols for talking to non OBDII vehicles.
It is configured by default to use a generic serial to ISO9141 converter such as those used on the VAG-COM software, It also supports smart interfaces from Multiplex Engineering and from B.Roadman for SAEJ1850 vehicles (mainly Ford and GM).
Please refer to the "supported interfaces" document to determine which interface support what protocols.